HPV DNA fragments – a silent Toll-like receptor 9 agonist in Gardasil® 9 – Sin Hang Lee, MD



Slides:

HPV vaccines depend on using proprietary aluminum adjuvants to enhance antibody production in the host because purified HPV L1 capsid proteins which are the active ingredients of HPV vaccines fail to stimulate antibody production effectively. However, the mechanisms of action of aluminum salts used as vaccine adjuvant still remain largely unknown and controversial. Recent progress in vaccine and immunology research has led us to believe that pattern recognition receptors (PRRs) of the innate immune system, particularly Toll-like receptors are the key modulators and may control the generation of humoral and cellular immune responses. In Cervarix® vaccine formulation, a Toll-like receptor 4 agonist, namely 3-0-descyl-4′-monophosphoryl lipid A (MPL), a derivative of bacterial lipopolysaccharide, which is bound to an aluminum salt, is used to enhance the initiation of the immune response through the activation of innate immunity, leading to an improved cellular and humoral adaptive immune response. In Gardasil® formulation, however, no Toll-like receptor agonist is listed. At the request of some of the parents whose children have developed serious adverse reactions after Gardasil® vaccination, our laboratory has tested samples of Gardasil 4 and Gardasil 9 supplied by these parents for the presence of HPV L1 gene DNA fragments in the vaccines since these HPV DNA fragments bound to the proprietary Amorphous Aluminum Hydroxyphosphate Sulfate (AAHS) adjuvant may serve as potent Toll-like receptor 9 agonist to augment the activation of innate immunity, including the production of large amount of pro-inflammatory cytokines. Overproduction of pro-inflammatory cytokines may cause tissue damages in certain genetically or physically predisposed individuals. This presentation focuses on polymerase chain reaction (PCR) and Sanger sequencing evidence for the presence of HPV L1 gene DNA fragments in non-B conformations which are bound to AAHS in the new vaccine Gardasil 9. Since the safety of Toll-like receptor 9 agonists as adjuvant in preventive vaccination has not been extensively studied, adequate informed consent is warranted when mass Gardasil 9 vaccination is being promoted as a tool for cervical cancer prevention.

Sin Hang Lee, MD

source: https://marishka-moi.com/

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